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FAM Isothiocyanate
Catalog No. CWI1003
| PACKING UNIT | price |
Lead time |
|---|---|---|
| 25 mg | $ |
Description
FAM Isothiocyanate is a reactive form of bright green dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 492/519 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. Isothiocyanates are moderately reactive but quite stable in water and most organic solvents. Isothiocyanates react to primary amine to form reasonably stable thiourea linkage. Whereas labeling of protein with NHS esters can typically be done at pH 8.3, conjugation for isothiocyanates usually require pH above 9. We offer FAM isothiocyanate for labeling of antibodies, peptides, proteins and nucleotide.
Citation & Reference
1. Rodrigues AF. Engineering graphene-based electrodes for optical neural stimulation. Nanoscale. 2023;15(2):687–706.
2. Tan X, Integrin and syndecan binding peptide-conjugated alginate hydrogel for modulation of Nucleus Pulposus Cell phenotype. Biomaterials. 2021 Oct;277:121113.
3. Kim HI, Biomolecular Imaging of colorectal tumor lesions using a FITC-labeled scfv-cκ fragment antibody. Scientific Reports. 2021 Aug 25;11(1).
4. Jain E, Copper-free azide–alkyne cycloaddition for peptide modification of alginate hydrogels. ACS Applied Bio Materials. 2021 Feb 2;4(2):1229–37.
5. Kim J-Y, Antifungal effect of a chimeric peptide HN-MC against pathogenic fungal strains. Antibiotics. 2020 Jul 28;9(8):454.
6. Park S-C, Functional mechanisms underlying the antimicrobial activity of the Oryza sativa TRX-like protein. International Journal of Molecular Sciences. 2019 Mar 20;20(6):1413.
7. Kim J-Y, Enhanced antifungal activity of engineered proteins via swapping between thioredoxin H2 and H3. Applied Sciences. 2019 Nov 8;9(22):4766.
8. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.
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