FAM Thiol

Catalog No. CWT1003
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1 mg $121.00
5 mg $279.00
25 mg $552.00
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Description

FAM Thiol is inactive form of bright green fluorescent dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 494/520 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. FAM thiol can be labeled to biomolecules through a disulfide bond formation with thiol of cysteine residue or can be utilized as a reference standard for dye-conjugates. 

Specifications

Fluorophore: FAM

Functional group: Thiol

Excitation/Emission Max.(nm): 494/520 

Spectrally similar dyes: Alexa488, Cy2

Extinction coefficient: ≥ 60,000 cm-1M-1

CF280: 0.18

Appearance: Yellow Solid

Molecular Weight: 437.47 g/mol    

Solubility: DMF, DMSO

Storage conditions: -20 ℃, protect from light







Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.


2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.


3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).


4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.


5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.

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