FAM Amine

Catalog No. CWE1003
PACKING UNIT

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1 mg $121.00
5 mg $279.00
25 mg $552.00
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Description

FAM Amine is inactive form of bright green fluorescent dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 494/520 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. The primary amine is attached to the fluorophore through an ethylamino linkage. FAM amine can be coupled with carboxylic acids at small molecules or on biomolecules by standard amide bond coupling conditions or can be utilized as a reference standard for dye-conjugates.  

Specifications

Fluorophore: FAM

Functional group: Primary amine

Excitation/Emission Max.(nm): 494/520

Spectrally similar dyes: Alexa488, Cy2

Extinction coefficient: ≥ 52,000 cm-1M-1

CF280: 0.2

Appearance: Yellow Solid

Molecular Weight: 420 g/mol    

Solubility: DMSO

Storage conditions: 4 ℃, protect from light




Citation & Reference

1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.


2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.


3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).


4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.


5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.

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