ROS Detection Probes

Hydrogen peroxide (H₂O₂) plays important roles in various cellular signal transductions in physiological processes as well as pathological effects. It has shown that H₂O₂ is the main reactive oxygen species (ROS) in redox signaling during cell proliferation and apoptotic process. Thus, quantitative analysis of endogenous H₂O₂ might to be a useful tool for understanding cell physiology. BioActs developed a ROS reactive fluorescence probe NpFlamma^® ROS 380 as an efficient detecting tool for fast, sensitive and selective imaging of low level H₂O₂ generated during various cellular processes.

NpFlamma^® ROS 380 comprised of a physical assembly of H₂O₂ reactive fluorophore and catalytic additive into an amphiphilic stabilization colloid. This versatile reactive nanoparticle is hardly fluorescent in inactivated state yet displays a strong red-shifted emission upon oxidized by H₂O₂. The maxima of Excitation/emission are at 380/450 nm. Since the emission is achieved by a chemical reaction, the interference between background fluorescence and the excitation light irradiation cannot occur. NpFlamma^® ROS 380 allows a real-time imaging of low-level H₂O₂ involved in cellular processes along with oxidative stress. We offer NpFlamma^® ROS 380 as a quantitative H₂O₂ selective fluorescence probe for biological and physiological analysis involving oxidative cell processes.