Description

FAM NHS ester is a reactive form of bright green dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 494/520 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. NHS esters readily react with amine-modified oligonucleotides or amino groups of proteins, i.e. the ε-amino groups of lysine or the amine terminus of nucleotides to form a chemically stable amide bond between dye and the biomolecule. We offer FAM NHS ester for labeling of antibodies, peptides, proteins and nucleotide. 

Specifications

Fluorophore: FAM

Reactive group: NHS ester

Excitation/Emission Max.(nm): 494/520 

Spectrally similar dyes: Alexa488, Cy2

Extinction coefficient: ≥ 64,000 cm-1M-1

CF280: 0.3

Appearance: Orange Solid

Molecular Weight: 473.39 g/mol   

Solubility: DMF

Storage conditions: -20 ℃, protect from light




Citation & Reference

1. Rodrigues AF. Engineering graphene-based electrodes for optical neural stimulation. Nanoscale. 2023;15(2):687–706.


2. Tan X, Integrin and syndecan binding peptide-conjugated alginate hydrogel for modulation of Nucleus Pulposus Cell phenotype. Biomaterials. 2021 Oct;277:121113. 


3. Kim HI, Biomolecular Imaging of colorectal tumor lesions using a FITC-labeled scfv-cκ fragment antibody. Scientific Reports. 2021 Aug 25;11(1). 


4. Jain E, Copper-free azide–alkyne cycloaddition for peptide modification of alginate hydrogels. ACS Applied Bio Materials. 2021 Feb 2;4(2):1229–37. 


5. Kim J-Y, Antifungal effect of a chimeric peptide HN-MC against pathogenic fungal strains. Antibiotics. 2020 Jul 28;9(8):454. 


6. Park S-C,  Functional mechanisms underlying the antimicrobial activity of the Oryza sativa TRX-like protein. International Journal of Molecular Sciences. 2019 Mar 20;20(6):1413. 


7. Kim J-Y, Enhanced antifungal activity of engineered proteins via swapping between thioredoxin H2 and H3. Applied Sciences. 2019 Nov 8;9(22):4766. 


8. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.


 

 

OPTION

Added cart

장바구니 아이콘

We put the items
in the shopping cart.