Description

Goat anti-rabbit IgG, HRP is a horseradish peroxidase (HRP) conjugated secondary antibody. HRP is resistance to auto-oxidation yet oxidized by H2O2 to produce characteristic fluorescence emission. Anti-rabbit secondary antibodies display specificity for rabbit IgG and are useful for the detection of specific target. Since multiple secondary antibodies can bind to a single primary antibody, Goat anti-rabbit IgG, HRP enables to provide the great sensitivity in signal amplification, to visualize low abundant targets and to reduce experimental time. We offer Goat anti-rabbit IgG, HRP as an activatable probe for various biological applications such as immunohistochemistry, ELISA, dot blot, Western blot, etc.


Applications

Notes

Western Blot (WB)

1:5,000-1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

1:500-1:5,000


Specifications

Conjugate: HRP

Species reactivity: Rabbit

Target: IgG

Host/Isotype: Goat/IgG

Type: Secondary antibody

Antibody form: Whole antibody

Form: Liquid

Concentration: 1 mg/mL

Storage buffer: PBS (pH 7.4)    

Contains: 15 mg/mL BSA

Storage conditions: 4 ℃, protect from light

Citation & Reference

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3. Choi Y-J, Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/AKT/mTOR pathway. Food Science and Human Wellness. 2024 May;13(3):1698–710. 


4. Yang Y, Ziziphus jujuba Miller ethanol extract restores disrupted intestinal barrier function via tight junction recovery and reduces inflammation. Antioxidants. 2024 May 7;13(5):575. 


5. Fan M, Use of a silkworm (Bombyx mori) larvae by-product for the treatment of atopic dermatitis: Inhibition of NF-ΚB nuclear translocation and MAPK signaling. Nutrients. 2023 Apr 5;15(7):1775. 


6. Choi Y-J,  salvia miltiorrhiza bunge ameliorates benign prostatic hyperplasia through regulation of oxidative stress via NRF-2/HO-1 activation. Journal of Microbiology and Biotechnology. 2023 Oct 31;34(5):1059–72. 


7. Jang JH, Rainbow trout USP4 downregulates LPS-induced inflammation by removing the K63-linked ubiquitin chain on TAK1. Fish & Shellfish Immunology. 2022 Dec;131:1019–26.


8. Park MG, DMSO-tolerant ornithine decarboxylase (ODC) tandem assay optimised for high-throughput screening. Journal of Enzyme Inhibition and Medicinal Chemistry. 2022 Nov 30;38(1):309–18. 


9. Park MG, High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays. Journal of Enzyme Inhibition and Medicinal Chemistry. 2021 Jan 1;36(1):2016–24.


10. Lee, Cheol-Jung. Magnolin inhibits cell migration and invasion by targeting the ERKs/RSK2 signaling pathway. BMC cancer 15.1 (2015): 1.


11. Agustina, Lia. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC molecular biology 15.1 (2014): 1.

 

12. Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans. Rohner S,Kalck V,Wang X,Ikegami K,Lieb JD,Gasser SM,Meister P The Journal of cell biology (200:589) 

 

13. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). Moravec R,Conger KK,D'Souza R,Allison AB,Casanova JE The Journal of biological chemistry (287:31138)

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