Description

Goat anti-rabbit IgG, HRP is a horseradish peroxidase (HRP) conjugated secondary antibody. HRP is resistance to auto-oxidation yet oxidized by H2O2 to produce characteristic fluorescence emission. Anti-rabbit secondary antibodies display specificity for rabbit IgG and are useful for the detection of specific target. Since multiple secondary antibodies can bind to a single primary antibody, Goat anti-rabbit IgG, HRP enables to provide the great sensitivity in signal amplification, to visualize low abundant targets and to reduce experimental time. We offer Goat anti-rabbit IgG, HRP as an activatable probe for various biological applications such as immunohistochemistry, ELISA, dot blot, Western blot, etc.


Applications

Notes

Western Blot (WB)

1:5,000-1:100,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

1:500-1:5,000


Specifications

Conjugate: HRP

Species reactivity: Rabbit

Target: IgG

Host/Isotype: Goat/IgG

Type: Secondary antibody

Antibody form: Whole antibody

Form: Liquid

Concentration: 1 mg/mL

Storage buffer: PBS (pH 7.4)    

Contains: 15 mg/mL BSA

Storage conditions: 4 ℃, protect from light

Citation & Reference

1. Agustina, Lia. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC molecular biology 15.1 (2014): 1.


2. Lee, Cheol-Jung. Magnolin inhibits cell migration and invasion by targeting the ERKs/RSK2 signaling pathway. BMC cancer 15.1 (2015): 1.

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