Description

Goat anti-rabbit IgG, FSD™ 680 is a fluorescence conjugated secondary antibody that displays excellent optical imaging with low cross reactivity. Anti-rabbit secondary antibodies display specificity for rabbit IgG and are useful for the detection of specific target. Since multiple secondary antibodies can bind to a single primary antibody, Goat anti-rabbit IgG, FSD™ 680 might provide the great sensitivity in signal amplification, visualize low abundant targets and reduce experimental time. FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. FSD™ 680 might be excited using 633 nm laser line and displays excellent optical property. We offer Goat anti-rabbit IgG, FSD™ 680 as a suitable fluorescent molecular probe for many biological experiments such as fluorescence microscopy, flow cytometry, microplate assays, protein and nucleic acid blots, in situ hybridization, etc.

 

 

Applications

Notes

Western Blot (WB)

0.1-0.4 µg/mL

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Flow Cytometry (Flow)

1-10 µg/mL

 

 

Specifications

Conjugate: FSD  680

Species reactivity: Rabbit

Target: IgG

Excitation/Emission Max.(nm): 679/696

Host/Isotype: Goat/IgG

Type: Secondary antibody

Antibody form: Whole antibody

Form: Liquid

Concentration: 2 mg/mL

Storage buffer: PBS (pH 7.4)    

Contains: 1.5% BSA, 5 mM sodium azide    

Storage conditions: 4 ℃, protect from light

Citation & Reference

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6. Choi Y-J,  salvia miltiorrhiza bunge ameliorates benign prostatic hyperplasia through regulation of oxidative stress via NRF-2/HO-1 activation. Journal of Microbiology and Biotechnology. 2023 Oct 31;34(5):1059–72. 


7. Jang JH, Rainbow trout USP4 downregulates LPS-induced inflammation by removing the K63-linked ubiquitin chain on TAK1. Fish & Shellfish Immunology. 2022 Dec;131:1019–26.


8. Park MG, DMSO-tolerant ornithine decarboxylase (ODC) tandem assay optimised for high-throughput screening. Journal of Enzyme Inhibition and Medicinal Chemistry. 2022 Nov 30;38(1):309–18. 


9. Park MG, High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays. Journal of Enzyme Inhibition and Medicinal Chemistry. 2021 Jan 1;36(1):2016–24.


10. Lee, Cheol-Jung. Magnolin inhibits cell migration and invasion by targeting the ERKs/RSK2 signaling pathway. BMC cancer 15.1 (2015): 1.


11. Agustina, Lia. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC molecular biology 15.1 (2014): 1.

 

12. Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans. Rohner S,Kalck V,Wang X,Ikegami K,Lieb JD,Gasser SM,Meister P The Journal of cell biology (200:589) 

 

13. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). Moravec R,Conger KK,D'Souza R,Allison AB,Casanova JE The Journal of biological chemistry (287:31138)

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