Description

Goat anti-mouse IgG, HRP is a horseradish peroxidase (HRP) conjugated secondary antibody. HRP is resistance to auto-oxidation yet oxidized by H2O2 to produce characteristic fluorescence emission. Anti-mouse secondary antibodies display specificity for mouse IgG and are useful for the detection of specific target. Since multiple secondary antibodies can bind to a single primary antibody, Goat anti-mouse IgG, HRP enables to provide the great sensitivity in signal amplification, to visualize low abundant targets and to reduce experimental time. We offer Goat anti-mouse IgG, HRP as an activatable probe for various biological applications such as immunohistochemistry, ELISA, dot blot, Western blot, etc.


Applications

Notes

Western Blot (WB)

1:5,000-1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

1:500-1:5,000


Specifications

Conjugate: HRP

Species reactivity: Mouse

Target: IgG

Host/Isotype: Goat/IgG

Type: Secondary antibody

Antibody form: Whole antibody

Form: Liquid

Concentration: 1 mg/mL

Storage buffer: PBS (pH 7.4)    

Contains: 15 mg/mL BSA

Storage conditions: 4 ℃, protect from light

Citation & Reference

1. Seo J, Ethanolic extract of bergenia purpurascens exhibits antimelanogenic effects in B16F10 cells through multiple mechanisms that suppress tyrosinase. Journal of Agricultural and Food Chemistry. 2024 Oct 19;72(43):23957–68.

 

2. Choi Y-J, Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/AKT/mTOR pathway. Food Science and Human Wellness. 2024 May;13(3):1698–710. 

 

3. Choi Y-J,  salvia miltiorrhiza bunge ameliorates benign prostatic hyperplasia through regulation of oxidative stress via NRF-2/HO-1 activation. Journal of Microbiology and Biotechnology. 2023 Oct 31;34(5):1059–72. 


4. Fan M, Use of a silkworm (Bombyx mori) larvae by-product for the treatment of atopic dermatitis: Inhibition of NF-ΚB nuclear translocation and MAPK signaling. Nutrients. 2023 Apr 5;15(7):1775. 

 

5. Jang JH, Rainbow trout USP4 downregulates LPS-induced inflammation by removing the K63-linked ubiquitin chain on TAK1. Fish & Shellfish Immunology. 2022 Dec;131:1019–26.

 

6. Yang Y, Ziziphus jujuba Miller ethanol extract restores disrupted intestinal barrier function via tight junction recovery and reduces inflammation. Antioxidants. 2024 May 7;13(5):575. 

 

7.  Moon KM, Docosatrienoic acid inhibits melanogenesis partly through suppressing the intracellular MITF/tyrosinase axis. Pharmaceuticals. 2024 Sept 11;17(9):1198.

 

8. Choi Y-J, salvia miltiorrhiza bunge ameliorates benign prostatic hyperplasia through regulation of oxidative stress via NRF-2/HO-1 activation. Journal of Microbiology and Biotechnology. 2023 Oct 31;34(5):1059–72.

 

9. Fan M, Use of a silkworm (Bombyx mori) larvae by-product for the treatment of atopic dermatitis: Inhibition of NF-ΚB nuclear translocation and MAPK signaling. Nutrients. 2023 Apr 5;15(7):1775. 

 

10.  Seong C, FOXO1 controls redox regulation and cellular physiology of BV-2 microglial cells. Inflammation. 2022 Dec 14;46(2):752–62. 

 

11. Lee M-K,  Brassinin abundant in Brassicaceae suppresses melanogenesis through dual mechanisms of tyrosinase inhibition. Foods. 2022 Dec 26;12(1):121.

 

12. Kang M,  quadrella incana (Capparaceae) leaf extract enhances proliferation and maintenance of neural stem/progenitor cells through upregulating glycolytic flux and redox potential. Oxidative Medicine and Cellular Longevity. 2020 Apr 27;2020:1–12. 

 

13. Lee, Cheol-Jung. Magnolin inhibits cell migration and invasion by targeting the ERKs/RSK2 signaling pathway. BMC cancer 15.1 (2015): 1.


14. Agustina, Lia. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC molecular biology 15.1 (2014): 1.

 

15. Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans. Rohner S,Kalck V,Wang X,Ikegami K,Lieb JD,Gasser SM,Meister P The Journal of cell biology (200:589) 

 

16. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). Moravec R,Conger KK,D'Souza R,Allison AB,Casanova JE The Journal of biological chemistry (287:31138)

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