Description

Flamma® Fluors 774 Maleimide is a thiol reactive near infrared (NIR) fluorescent dye that used to generate a stable fluorescence signal in bioimaging The maxima of Ex/Em values are at 774/800 nm, similar to that of IRDye 800, Cy7.5 and CF770. Flamma 774 might be excited using 750 nm laser line or dye-pumped laser excitation and the emission occurs at NIR region. Flamma Fluors 774 is ideal for protein, antibody and nucleic acid labeling for in vitro imaging and other fluorescence detection methods. Maleimides selectively label thiols of cysteine residue via 1,4-addition pathway, without interacting with amines, to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they might react with primary amines under strong basic environment. We offer Flamma Fluors 774 Maleimide for labeling of antibodies, peptides, proteins, ligands and in vivo NIR imaging. 

Specifications

Fluorophore: Flamma® Fluors 774

Reactive group: Maleimide

Excitation/Emission Max.(nm): 774/800

Spectrally similar dyes: Cy7.5, DyLight800, IRDye800

Extinction coefficient: ≥ 182,000 cm-1M-1

CF280: 0.1

Appearance: Green Solid

Molecular Weight: 1051.19 g/mol      

Solubility: DMF, DMSO

Storage conditions: -20 ℃, protect from light

Citation & Reference

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