Description

 Biotin, Flamma® 648 is a fluorescent biotin that Flamma® 648 dye is covalently attached to biotin. Biotin, a 244 dalton vitamin found in all living cells, binds to avidin and streptavidin in high affinity. In biotechnology, biotin conjugated antibodies, enzymes, small molecules or reporters are used to form complex with avidin/streptavidin. Flamma® 648 is a far-red fluorescent dye. Flamma®648 might be excited using 593 or 633 nm laser line. Fluorescence–conjugated biotins have been utilized in various applications such as cell surface labeling, ELISA, immunohistochemistry, affinity purification, FACS, EMSA, etc. The bond formation between biotin and avidin/streptavidin is rapid, and the binding is stable at high temperature, in a wide range of pH and denaturing agents. BioActs offers Biotin, Flamma® 648 as an effective detecting and analytic probe for diverse applications in medical diagnostics, in vitro, in vivo and animal model study.

Specifications

Fluorophore label: Flamma® 648

Reactive group: Biotin

Reacting toward: Avidin / Streptavidin

Excitation/Emission Max.(nm): 648/663

Molecular weight: 927.20 g/mol

Storage conditions: 4 ℃, protect from light

Citation & Reference

 

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